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ul44  (Biosynth Carbosynth)


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    Biosynth Carbosynth ul44
    Figure 2. Viral gene expression and protein analysis TB40E WT, TB40E ∆RNA1.2 half, and TB40E ∆RNA1.2. (A) HF cells were infected with TB40E WT, ∆RNA1.2 half, and ∆RNA1.2 (MOI = 1). Cell lysate was harvested at 4 hpi, 24 hpi, 48 hpi, and 72 hpi. RNA was isolated, and transcript expression was measured by qPCR analysis using primers and probes specific to RNA1.2, RNA4.9, RNA B2.7, IE2, UL54, UL86 and 7SK (cellular). Fold change of each transcript was calculated using ∆∆CT, normalized with cellular 7SK and compared to the 4 hpi time point. Error bars indicate S.D. from independent experiments (N = 3). Statistical analysis was performed using two-way ANOVA, ****, t-test; n.s., not significant. (B) For protein analysis, cells were infected as described above. The mock and infected cell lysate was harvested at 24 hpi and 72 hpi. Cell lysate was denatured, and proteins were separated by SDS PAGE and transferred to PVDF membrane for Western blot. Proteins were identified using antibodies specific for labeling IE2, IE1, pp65, <t>UL44,</t> and H3.
    Ul44, supplied by Biosynth Carbosynth, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ul44/pm40006904-147-12-14?v=Biosynth+Carbosynth
    Average 93 stars, based on 3 article reviews
    ul44 - by Bioz Stars, 2026-07
    93/100 stars

    Images

    1) Product Images from "Characterization of Human Cytomegalovirus (HCMV) Long Non-Coding RNA1.2 During Lytic Replication."

    Article Title: Characterization of Human Cytomegalovirus (HCMV) Long Non-Coding RNA1.2 During Lytic Replication.

    Journal: Viruses

    doi: 10.3390/v17020149

    Figure 2. Viral gene expression and protein analysis TB40E WT, TB40E ∆RNA1.2 half, and TB40E ∆RNA1.2. (A) HF cells were infected with TB40E WT, ∆RNA1.2 half, and ∆RNA1.2 (MOI = 1). Cell lysate was harvested at 4 hpi, 24 hpi, 48 hpi, and 72 hpi. RNA was isolated, and transcript expression was measured by qPCR analysis using primers and probes specific to RNA1.2, RNA4.9, RNA B2.7, IE2, UL54, UL86 and 7SK (cellular). Fold change of each transcript was calculated using ∆∆CT, normalized with cellular 7SK and compared to the 4 hpi time point. Error bars indicate S.D. from independent experiments (N = 3). Statistical analysis was performed using two-way ANOVA, ****, t-test; n.s., not significant. (B) For protein analysis, cells were infected as described above. The mock and infected cell lysate was harvested at 24 hpi and 72 hpi. Cell lysate was denatured, and proteins were separated by SDS PAGE and transferred to PVDF membrane for Western blot. Proteins were identified using antibodies specific for labeling IE2, IE1, pp65, UL44, and H3.
    Figure Legend Snippet: Figure 2. Viral gene expression and protein analysis TB40E WT, TB40E ∆RNA1.2 half, and TB40E ∆RNA1.2. (A) HF cells were infected with TB40E WT, ∆RNA1.2 half, and ∆RNA1.2 (MOI = 1). Cell lysate was harvested at 4 hpi, 24 hpi, 48 hpi, and 72 hpi. RNA was isolated, and transcript expression was measured by qPCR analysis using primers and probes specific to RNA1.2, RNA4.9, RNA B2.7, IE2, UL54, UL86 and 7SK (cellular). Fold change of each transcript was calculated using ∆∆CT, normalized with cellular 7SK and compared to the 4 hpi time point. Error bars indicate S.D. from independent experiments (N = 3). Statistical analysis was performed using two-way ANOVA, ****, t-test; n.s., not significant. (B) For protein analysis, cells were infected as described above. The mock and infected cell lysate was harvested at 24 hpi and 72 hpi. Cell lysate was denatured, and proteins were separated by SDS PAGE and transferred to PVDF membrane for Western blot. Proteins were identified using antibodies specific for labeling IE2, IE1, pp65, UL44, and H3.

    Techniques Used: Gene Expression, Infection, Isolation, Expressing, SDS Page, Membrane, Western Blot, Labeling

    Figure 3. Viral protein localization is comparable between TB40E WT, TB40E ∆RNA1.2 half, and TB40E ∆RNA1.2. HF cells were infected with TB40E WT, TB40E ∆RNA1.2 half, and TB40E ∆RNA1.2 (MOI = 1). After 72 hpi, mock and infected cells were fixed and permeabilized. Cells were labeled with antibodies to detect viral proteins UL44, IE2, UL57, and UL84 (Red). Nuclei were stained with DAPI (blue). GFP expression can be detected in infected cells (green). Cells were mounted and imaged at 63x. Zoom panels are indicated on the right to show viral protein localization patterns.
    Figure Legend Snippet: Figure 3. Viral protein localization is comparable between TB40E WT, TB40E ∆RNA1.2 half, and TB40E ∆RNA1.2. HF cells were infected with TB40E WT, TB40E ∆RNA1.2 half, and TB40E ∆RNA1.2 (MOI = 1). After 72 hpi, mock and infected cells were fixed and permeabilized. Cells were labeled with antibodies to detect viral proteins UL44, IE2, UL57, and UL84 (Red). Nuclei were stained with DAPI (blue). GFP expression can be detected in infected cells (green). Cells were mounted and imaged at 63x. Zoom panels are indicated on the right to show viral protein localization patterns.

    Techniques Used: Infection, Labeling, Staining, Expressing

    Figure 9. Detection of RNA1.2 interacting with viral and cellular proteins. HF cells were infected with TB40E. After 72 hpi, cell lysate was fixed and sheared. An RNA-IP was performed using antibodies to detect IgG, H2A, RECQ1, and UL44. RNA was isolated, and a qPCR analysis was performed to detect the RNA bound to protein for the IPs. In the qPCR analysis, the primers and probes used to detect transcripts were RNA1.2 and GAPDH. Percent input was calculated and graphed (N = 3). Error bars indicate the standard deviation from the mean. Statistical analysis was performed using Student’s t-test with multiple comparisons *, p < 0.05.
    Figure Legend Snippet: Figure 9. Detection of RNA1.2 interacting with viral and cellular proteins. HF cells were infected with TB40E. After 72 hpi, cell lysate was fixed and sheared. An RNA-IP was performed using antibodies to detect IgG, H2A, RECQ1, and UL44. RNA was isolated, and a qPCR analysis was performed to detect the RNA bound to protein for the IPs. In the qPCR analysis, the primers and probes used to detect transcripts were RNA1.2 and GAPDH. Percent input was calculated and graphed (N = 3). Error bars indicate the standard deviation from the mean. Statistical analysis was performed using Student’s t-test with multiple comparisons *, p < 0.05.

    Techniques Used: Infection, Isolation, Standard Deviation



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    Figure 2. Viral gene expression and protein analysis TB40E WT, TB40E ∆RNA1.2 half, and TB40E ∆RNA1.2. (A) HF cells were infected with TB40E WT, ∆RNA1.2 half, and ∆RNA1.2 (MOI = 1). Cell lysate was harvested at 4 hpi, 24 hpi, 48 hpi, and 72 hpi. RNA was isolated, and transcript expression was measured by qPCR analysis using primers and probes specific to RNA1.2, RNA4.9, RNA B2.7, IE2, UL54, UL86 and 7SK (cellular). Fold change of each transcript was calculated using ∆∆CT, normalized with cellular 7SK and compared to the 4 hpi time point. Error bars indicate S.D. from independent experiments (N = 3). Statistical analysis was performed using two-way ANOVA, ****, t-test; n.s., not significant. (B) For protein analysis, cells were infected as described above. The mock and infected cell lysate was harvested at 24 hpi and 72 hpi. Cell lysate was denatured, and proteins were separated by SDS PAGE and transferred to PVDF membrane for Western blot. Proteins were identified using antibodies specific for labeling IE2, IE1, pp65, <t>UL44,</t> and H3.
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    Image Search Results


    List of antibodies used <xref ref-type= a " width="100%" height="100%">

    Journal: Journal of Virology

    Article Title: RAF1 promotes successful human cytomegalovirus replication and is regulated by AMPK-mediated phosphorylation during infection

    doi: 10.1128/jvi.01866-24

    Figure Lengend Snippet: List of antibodies used a

    Article Snippet: UL44 , Virusys Corporation , CA006.

    Techniques:

    Figure 2. Viral gene expression and protein analysis TB40E WT, TB40E ∆RNA1.2 half, and TB40E ∆RNA1.2. (A) HF cells were infected with TB40E WT, ∆RNA1.2 half, and ∆RNA1.2 (MOI = 1). Cell lysate was harvested at 4 hpi, 24 hpi, 48 hpi, and 72 hpi. RNA was isolated, and transcript expression was measured by qPCR analysis using primers and probes specific to RNA1.2, RNA4.9, RNA B2.7, IE2, UL54, UL86 and 7SK (cellular). Fold change of each transcript was calculated using ∆∆CT, normalized with cellular 7SK and compared to the 4 hpi time point. Error bars indicate S.D. from independent experiments (N = 3). Statistical analysis was performed using two-way ANOVA, ****, t-test; n.s., not significant. (B) For protein analysis, cells were infected as described above. The mock and infected cell lysate was harvested at 24 hpi and 72 hpi. Cell lysate was denatured, and proteins were separated by SDS PAGE and transferred to PVDF membrane for Western blot. Proteins were identified using antibodies specific for labeling IE2, IE1, pp65, UL44, and H3.

    Journal: Viruses

    Article Title: Characterization of Human Cytomegalovirus (HCMV) Long Non-Coding RNA1.2 During Lytic Replication.

    doi: 10.3390/v17020149

    Figure Lengend Snippet: Figure 2. Viral gene expression and protein analysis TB40E WT, TB40E ∆RNA1.2 half, and TB40E ∆RNA1.2. (A) HF cells were infected with TB40E WT, ∆RNA1.2 half, and ∆RNA1.2 (MOI = 1). Cell lysate was harvested at 4 hpi, 24 hpi, 48 hpi, and 72 hpi. RNA was isolated, and transcript expression was measured by qPCR analysis using primers and probes specific to RNA1.2, RNA4.9, RNA B2.7, IE2, UL54, UL86 and 7SK (cellular). Fold change of each transcript was calculated using ∆∆CT, normalized with cellular 7SK and compared to the 4 hpi time point. Error bars indicate S.D. from independent experiments (N = 3). Statistical analysis was performed using two-way ANOVA, ****, t-test; n.s., not significant. (B) For protein analysis, cells were infected as described above. The mock and infected cell lysate was harvested at 24 hpi and 72 hpi. Cell lysate was denatured, and proteins were separated by SDS PAGE and transferred to PVDF membrane for Western blot. Proteins were identified using antibodies specific for labeling IE2, IE1, pp65, UL44, and H3.

    Article Snippet: Membrane with samples from 72 hpi was probed with antibodies to detect UL44 1:1000 (Fitzgerald, 10-C50I), pp65 1:150 (Abcam, ab49214), and H3.

    Techniques: Gene Expression, Infection, Isolation, Expressing, SDS Page, Membrane, Western Blot, Labeling

    Figure 3. Viral protein localization is comparable between TB40E WT, TB40E ∆RNA1.2 half, and TB40E ∆RNA1.2. HF cells were infected with TB40E WT, TB40E ∆RNA1.2 half, and TB40E ∆RNA1.2 (MOI = 1). After 72 hpi, mock and infected cells were fixed and permeabilized. Cells were labeled with antibodies to detect viral proteins UL44, IE2, UL57, and UL84 (Red). Nuclei were stained with DAPI (blue). GFP expression can be detected in infected cells (green). Cells were mounted and imaged at 63x. Zoom panels are indicated on the right to show viral protein localization patterns.

    Journal: Viruses

    Article Title: Characterization of Human Cytomegalovirus (HCMV) Long Non-Coding RNA1.2 During Lytic Replication.

    doi: 10.3390/v17020149

    Figure Lengend Snippet: Figure 3. Viral protein localization is comparable between TB40E WT, TB40E ∆RNA1.2 half, and TB40E ∆RNA1.2. HF cells were infected with TB40E WT, TB40E ∆RNA1.2 half, and TB40E ∆RNA1.2 (MOI = 1). After 72 hpi, mock and infected cells were fixed and permeabilized. Cells were labeled with antibodies to detect viral proteins UL44, IE2, UL57, and UL84 (Red). Nuclei were stained with DAPI (blue). GFP expression can be detected in infected cells (green). Cells were mounted and imaged at 63x. Zoom panels are indicated on the right to show viral protein localization patterns.

    Article Snippet: Membrane with samples from 72 hpi was probed with antibodies to detect UL44 1:1000 (Fitzgerald, 10-C50I), pp65 1:150 (Abcam, ab49214), and H3.

    Techniques: Infection, Labeling, Staining, Expressing

    Figure 9. Detection of RNA1.2 interacting with viral and cellular proteins. HF cells were infected with TB40E. After 72 hpi, cell lysate was fixed and sheared. An RNA-IP was performed using antibodies to detect IgG, H2A, RECQ1, and UL44. RNA was isolated, and a qPCR analysis was performed to detect the RNA bound to protein for the IPs. In the qPCR analysis, the primers and probes used to detect transcripts were RNA1.2 and GAPDH. Percent input was calculated and graphed (N = 3). Error bars indicate the standard deviation from the mean. Statistical analysis was performed using Student’s t-test with multiple comparisons *, p < 0.05.

    Journal: Viruses

    Article Title: Characterization of Human Cytomegalovirus (HCMV) Long Non-Coding RNA1.2 During Lytic Replication.

    doi: 10.3390/v17020149

    Figure Lengend Snippet: Figure 9. Detection of RNA1.2 interacting with viral and cellular proteins. HF cells were infected with TB40E. After 72 hpi, cell lysate was fixed and sheared. An RNA-IP was performed using antibodies to detect IgG, H2A, RECQ1, and UL44. RNA was isolated, and a qPCR analysis was performed to detect the RNA bound to protein for the IPs. In the qPCR analysis, the primers and probes used to detect transcripts were RNA1.2 and GAPDH. Percent input was calculated and graphed (N = 3). Error bars indicate the standard deviation from the mean. Statistical analysis was performed using Student’s t-test with multiple comparisons *, p < 0.05.

    Article Snippet: Membrane with samples from 72 hpi was probed with antibodies to detect UL44 1:1000 (Fitzgerald, 10-C50I), pp65 1:150 (Abcam, ab49214), and H3.

    Techniques: Infection, Isolation, Standard Deviation